Digital PCR (dPCR) is a method for absolute quantification of nucleic acids. Unlike qPCR, dPCR does not rely on standard curves. The sample is partitioned into many individual chambers (8.5K or 2.6K) in a microfluidic chip. After partitioning, the reactions undergo end-point PCR cycling, and partitions are analyzed for the presence or absence of a fluorescent signal. The number of nucleic acid molecules is then calculated based on the principles of Poisson distribution. Quantitative PCR (qPCR) is a technique used to quantify specific DNA or RNA molecules present in a sample. It employs fluorescent dyes or probes to monitor the amplification products in real-time during the polymerase chain reaction (PCR) process, enabling quantitative analysis of target nucleic acids. This technique is widely used in fields such as gene expression analysis, virus detection, genotyping, and copy number variation analysis.