Before Sequencing
| Please follow the instructions below |
|---|
| 1. Except for bacterial cells, please attach DNA electrophoresis pictures for other samples and indicate the amount of sample in the picture. |
| 2. Except for Primer (T7、T3、SP6、M13F、M13R etc.), if you design primer yourself, please indicate the name and concentration (10 pmole/µl), and provide at least 5 µl for each reaction. |
| 3. Please dissolve Plasmid DNA or PCR product in ddH2O, and do not dissolve them in TE Buffer to avoid interference. |
| 4. For delivery of Plasmid DNA, the volume must be >10 µl and the concentration must be >40 ng/µl. |
| 5. For self-purified PCR products, the volume after purification must be >10 µl, and the concentration must be >20 ng/µl. |
| 6. After DNA sequencing is completed, the remaining DNA will be stored for one week and will be destroyed after the expiry date. |
Note : If the sample contains GC-rich, GT-rich, or similar sequences requiring special treatment, please assist by specifying this in the order. We utilize exclusive reagents from the ABI original manufacturer to address complex structural issues of the sample.
★To learn more about issues related to Sanger Sequencing, please click on the link Sanger Sequencing FAQs .
★If you would like to entrust Sanger Sequencing service, please click on the Laboratory Login. After logging in, you can proceed with the order.
★If you need to download the sample testing service order form for Sanger Sequencing, please click on the DOWNLOAD button.
Primer list
| Name | Length | Oligo Sequence |
|---|---|---|
| 3'AOX | 21 | GCAAATGGCATTCTGACATCC |
| 3'DNA-BD | 24 | TAAGAGTCACTTTAAAATTTGTAT |
| 3'AD | 20 | AGATGGTGCACGATGCACAG |
| 5'AOX | 21 | GACTGGTTCCAATTGACAAGC |
| ACGFP1-R | 20 | GGTGGTGCAGATGAACTTCA |
| ACYCDuetUp1 | 19 | GGATCTCGACGCTCTCCCT |
| ADH1-TTR | 22 | GTAAATTTCTGGCAAGGTAGAC |
| AS S¡ETag 18 | 18 | GTCCATGTGCTGGCGTTC |
| BAD-F | 23 | GCTATGCCATAGCATTTTTATCC |
| BAD-R | 25 | GCGTTCTGATTTAATCTGTATCAGG |
| BGH-R | 18 | TAGAAGGCACAGTCGAGG |
| CL3 | 23 | GTTGATTGCAGTCCAGTTACGCT |
| CMV-F | 20 | ATGGGAGTTTGTTTTGGCAC |
| CMV24 | 21 | AGGCTGGTGGGCACTGGAGTG |
| CMVbeta-R | 20 | CAGACCAATGCCTCCCAGAC |
| CMV-IE | 18 | TGTACGGTGGGAGGAGGTCTA |
| ColiDOWN | 21 | TTCACTTCTGAGTTCGGCATG |
| DsRed-1211F | 20 | ACATCACCAACCACAACGAG |
| DsRed1-C | 24 | AGCTGGACATCACCTCCCACAACG |
| DsRed1-N | 21 | GTACTGGAACTGGGGGGACAG |
| DuetDown1 | 20 | GATTATGCGGCCGTGTACAA |
| DuetUp2 | 20 | TTGTACACGGCCGCATAATC |
| EGFP-3957F | 20 | TATAGTCCTGTCGGGTTTCG |
| EGFP-C | 22 | CATGGTCCTGCTGGAGTTCGTG |
| EGFP-CR | 21 | CAAACCACAACTAGAATGCAG |
| EGFP-N | 22 | CGTCGCCGTCCAGCTCGACCAG |
| GAL1 | 24 | AATATACCTCTATACTTTAACGTC |
| GAL4-ADF | 21 | TATAACGCGTTTGGAATCACT |
| GFP reverse | 22 | GCTACATACGGAAAGCTTACCC |
| GL1 | 24 | TGTATCTTATGGTACTGTAACTG |
| GL2 | 21 | CTTTATGTTTTTGGCGTCTTCC |
| HcRed1-1185F | 22 | CTTCCACTTCACCGACATCC |
| KAN-2 FP-1 | 25 | ACCTACAACAAAGCTCTCATCAACC |
| KAN-2 RP-1 | 25 | GCAATGTAACATCAGAGATTTTGAG |
| LKO-shRNA-R | 19 | CTGTTGCTATTATGTCTAC |
| M13F | 18 | TGTAAAACGACGGCCAGT |
| M13F(-40) | 17 | GTTTTCCCAGTCACGAC |
| M13R | 18 | ACAGGAAACAGCTATGAC |
| malE | 24 | GGTCGTCAGACTGTCGATGAAGCC |
| Mxe InteinR | 18 | GGCACGATGTAGGCGATG |
| N-CMV30 | 20 | AATGTCGTAATAACCCCGCCCCGTTGACGC |
| OplE2 Forward | 20 | CGCAACGATCTGGTAAACAC |
| PACT-F | 22 | CGAACCTCATAACAACTCAAAC |
| PBAD-myc-his-241F | 22 | ATCCTACCTGACGCTTTTTATC |
| pCMV | 27 | GATCCGGTACTAGAGGAACTGAAAAAC |
| pCMV-GIN-ZEO-F | 20 | CCGCTGTTTGAATGAGGCTT |
| pCMV-HA-R | 21 | CCCTGAACCTGAAACATAAAA |
| pDNR-M13R | 20 | CGAGGGAAACAGCTATGACC |
| pET Upstream | 16 | ATGCGTCCGGCGTAGA |
| pGEX3' | 23 | CCGGGAGCTGCATGTGTCAGAGG |
| pGEX5' | 23 | GGGCTGGCAAGCCACGTTTGGTG |
| PLKO.1-seg | 20 | CTATTCTTTCCCCTGCACTG |
| pMIR5' | 18 | AGGCGATTAAGTTGGGTA |
| PNRK-R | 19 | GCTCGAAAACAGCTATGAC |
| PNRK-R880 | 22 | CAAATAAAGCAATAGCATCACA |
| polyhedron F | 20 | AAATGATAACCATCTCGCAA |
| pQEF | 22 | GGCGTATCACGAGGCCCTTTCG |
| pQER | 22 | CATTACTGGATCTATCAACAGG |
| pRSET reverse | 21 | CTAGTTATTGCTCAGCGGTGG |
| pSE380F | 20 | AATCATCCGGCTCGTATAAT |
| pTrcHis forward | 21 | GAGGTATATATTAATGTATCG |
| pTrcHis reverse | 18 | GATTTAATCTGTATCAGG |
| RV3 | 20 | CTAGCAAAATAGGCTGTCCC |
| RV4 | 20 | GACGATAGTCATGCCCCGCG |
| S-DsbC-Tag | 17 | GAATTTCTCGACGAACA |
| S-tag | 17 | GAACGCCAGCACATGGACAGC |
| S-Tag-18 | 18 | GAACGCCAGCACATGGAC |
| SP6 | 18 | ATTTAGGTGACACTATAG |
| SR2 | 26 | GGTCAGGTATGATTTAAATGGTCAGT |
| T3 Promoter | 20 | ATTAACCCTCACTAAAGGGA |
| T7 Promoter Primer(T7-20) | 20 | TAATACGACTCACTATAGGG |
| T7 terminator | 19 | GCTAGTTATTGCTCAGCGG |
| T7-18 | 18 | TAATACGACTCACTATAG |
| T7-reverse | 21 | CTAGTTATTGCTCAACGGTGG |
| TA-F | 20 | CAAGGCGATTAAGTTGGGTA |
| TA-R | 20 | GGAATTGTGAGCGGATAACA |
| Trx-F | 18 | TTCCTCGACGCTAACCTG |
| Trx-R | 20 | TGTAAAACGACGGCCAGTGC |
| TrxFus forward | 18 | TTCCTCGACGCTAACCTG |
| U6 promoter | 19 | TACAAAATACGTGACGTAG |
| V5(C-term)reverse | 20 | ACCGAGGAGAGGGTTAGGGA |
| Xpress Forward | 19 | TATGGCTAGCATGACTGGT |
| α-factor | 18 | TATTGCCAGCATTGCTGC |
| XL39 | 20 | ATTAGGACAAGGCTGGTGGG |
| VP1.5 | 20 | GGACTTTCCAAAATGTCG |
| ITS4 | 20 | TCCTCCGCTTATTGATATGC |
| ITS1 | 19 | TCCGTAGGTGAACCTGCGG |
| ITS5 | 22 | GGAAGTAAAAGTCGTAACAAGG |
| 27F | 20 | AGAGTTTGATCMTGGCTCAG |
| 1492R | 22 | TACGGYTACCTTGTTACGACTT |
| 533F | 19 | GTGCCAGCAGCCGCGGTAA |
| 805R | 20 | GACTACCAGGGTATCTAATC |
★If there are Universal Primers not listed in the table, please contact our laboratory by phone or email for inquiries.
Strain Identification
Advantages of Strain Identification Service :
1. Genetic identification methods analyze bacterial 16S and fungal ITS, capable of identifying at the "species" level.
2. Both agar plates and liquid cultures of individual strains are applicable.
3. Reports are generated within 5 working days upon sample receipt.
4. A dedicated data management system is available for customers to download files.