Sanger Sequencing
The ABI 3730xl system yields sequencing results with an average length of approximately 700-800 base pairs, with a maximum length of up to 1000 base pairs. With an accuracy of 99.99%, it is capable of simultaneous sequencing reactions for 96 capillaries.
Before Sequencing Primer list

Before Sequencing

Please follow the instructions below
1. Except for bacterial cells, please attach DNA electrophoresis pictures for other samples and indicate the amount of sample in the picture.
2. Except for Primer (T7、T3、SP6、M13F、M13R etc.), if you design primer yourself, please indicate the name and concentration (10 pmole/µl), and provide at least 5 µl for each reaction.
3. Please dissolve Plasmid DNA or PCR product in ddH2O, and do not dissolve them in TE Buffer to avoid interference.
4. For delivery of Plasmid DNA, the volume must be >10 µl and the concentration must be >40 ng/µl.
5. For self-purified PCR products, the volume after purification must be >10 µl, and the concentration must be >20 ng/µl.
6. After DNA sequencing is completed, the remaining DNA will be stored for one week and will be destroyed after the expiry date.

Note : If the sample contains GC-rich, GT-rich, or similar sequences requiring special treatment, please assist by specifying this in the order. We utilize exclusive reagents from the ABI original manufacturer to address complex structural issues of the sample.

★To learn more about issues related to Sanger Sequencing, please click on the link Sanger Sequencing FAQs .
★If you would like to entrust Sanger Sequencing service, please click on the Laboratory Login. After logging in, you can proceed with the order.
★If you need to download the sample testing service order form for Sanger Sequencing, please click on the DOWNLOAD button.

Primer list

Name Length Oligo Sequence
3'AOX 21 GCAAATGGCATTCTGACATCC
3'DNA-BD 24 TAAGAGTCACTTTAAAATTTGTAT
3'AD 20 AGATGGTGCACGATGCACAG
5'AOX 21 GACTGGTTCCAATTGACAAGC
ACGFP1-R 20 GGTGGTGCAGATGAACTTCA
ACYCDuetUp1 19 GGATCTCGACGCTCTCCCT
ADH1-TTR 22 GTAAATTTCTGGCAAGGTAGAC
AS S¡ETag 18 18 GTCCATGTGCTGGCGTTC
BAD-F 23 GCTATGCCATAGCATTTTTATCC
BAD-R 25 GCGTTCTGATTTAATCTGTATCAGG
BGH-R 18 TAGAAGGCACAGTCGAGG
CL3 23 GTTGATTGCAGTCCAGTTACGCT
CMV-F 20 ATGGGAGTTTGTTTTGGCAC
CMV24 21 AGGCTGGTGGGCACTGGAGTG
CMVbeta-R 20 CAGACCAATGCCTCCCAGAC
CMV-IE 18 TGTACGGTGGGAGGAGGTCTA
ColiDOWN 21 TTCACTTCTGAGTTCGGCATG
DsRed-1211F 20 ACATCACCAACCACAACGAG
DsRed1-C 24 AGCTGGACATCACCTCCCACAACG
DsRed1-N 21 GTACTGGAACTGGGGGGACAG
DuetDown1 20 GATTATGCGGCCGTGTACAA
DuetUp2 20 TTGTACACGGCCGCATAATC
EGFP-3957F 20 TATAGTCCTGTCGGGTTTCG
EGFP-C 22 CATGGTCCTGCTGGAGTTCGTG
EGFP-CR 21 CAAACCACAACTAGAATGCAG
EGFP-N 22 CGTCGCCGTCCAGCTCGACCAG
GAL1 24 AATATACCTCTATACTTTAACGTC
GAL4-ADF 21 TATAACGCGTTTGGAATCACT
GFP reverse 22 GCTACATACGGAAAGCTTACCC
GL1 24 TGTATCTTATGGTACTGTAACTG
GL2 21 CTTTATGTTTTTGGCGTCTTCC
HcRed1-1185F 22 CTTCCACTTCACCGACATCC
KAN-2 FP-1 25 ACCTACAACAAAGCTCTCATCAACC
KAN-2 RP-1 25 GCAATGTAACATCAGAGATTTTGAG
LKO-shRNA-R 19 CTGTTGCTATTATGTCTAC
M13F 18 TGTAAAACGACGGCCAGT
M13F(-40) 17 GTTTTCCCAGTCACGAC
M13R 18 ACAGGAAACAGCTATGAC
malE 24 GGTCGTCAGACTGTCGATGAAGCC
Mxe InteinR 18 GGCACGATGTAGGCGATG
N-CMV30 20 AATGTCGTAATAACCCCGCCCCGTTGACGC
OplE2 Forward 20 CGCAACGATCTGGTAAACAC
PACT-F 22 CGAACCTCATAACAACTCAAAC
PBAD-myc-his-241F 22 ATCCTACCTGACGCTTTTTATC
pCMV 27 GATCCGGTACTAGAGGAACTGAAAAAC
pCMV-GIN-ZEO-F 20 CCGCTGTTTGAATGAGGCTT
pCMV-HA-R 21 CCCTGAACCTGAAACATAAAA
pDNR-M13R 20 CGAGGGAAACAGCTATGACC
pET Upstream 16 ATGCGTCCGGCGTAGA
pGEX3' 23 CCGGGAGCTGCATGTGTCAGAGG
pGEX5' 23 GGGCTGGCAAGCCACGTTTGGTG
PLKO.1-seg 20 CTATTCTTTCCCCTGCACTG
pMIR5' 18 AGGCGATTAAGTTGGGTA
PNRK-R 19 GCTCGAAAACAGCTATGAC
PNRK-R880 22 CAAATAAAGCAATAGCATCACA
polyhedron F 20 AAATGATAACCATCTCGCAA
pQEF 22 GGCGTATCACGAGGCCCTTTCG
pQER 22 CATTACTGGATCTATCAACAGG
pRSET reverse 21 CTAGTTATTGCTCAGCGGTGG
pSE380F 20 AATCATCCGGCTCGTATAAT
pTrcHis forward 21 GAGGTATATATTAATGTATCG
pTrcHis reverse 18 GATTTAATCTGTATCAGG
RV3 20 CTAGCAAAATAGGCTGTCCC
RV4 20 GACGATAGTCATGCCCCGCG
S-DsbC-Tag 17 GAATTTCTCGACGAACA
S-tag 17 GAACGCCAGCACATGGACAGC
S-Tag-18 18 GAACGCCAGCACATGGAC
SP6 18 ATTTAGGTGACACTATAG
SR2 26 GGTCAGGTATGATTTAAATGGTCAGT
T3 Promoter 20 ATTAACCCTCACTAAAGGGA
T7 Promoter Primer(T7-20) 20 TAATACGACTCACTATAGGG
T7 terminator 19 GCTAGTTATTGCTCAGCGG
T7-18 18 TAATACGACTCACTATAG
T7-reverse 21 CTAGTTATTGCTCAACGGTGG
TA-F 20 CAAGGCGATTAAGTTGGGTA
TA-R 20 GGAATTGTGAGCGGATAACA
Trx-F 18 TTCCTCGACGCTAACCTG
Trx-R 20 TGTAAAACGACGGCCAGTGC
TrxFus forward 18 TTCCTCGACGCTAACCTG
U6 promoter 19 TACAAAATACGTGACGTAG
V5(C-term)reverse 20 ACCGAGGAGAGGGTTAGGGA
Xpress Forward 19 TATGGCTAGCATGACTGGT
α-factor 18 TATTGCCAGCATTGCTGC
XL39 20 ATTAGGACAAGGCTGGTGGG
VP1.5 20 GGACTTTCCAAAATGTCG
ITS4 20 TCCTCCGCTTATTGATATGC
ITS1 19 TCCGTAGGTGAACCTGCGG
ITS5 22 GGAAGTAAAAGTCGTAACAAGG
27F 20 AGAGTTTGATCMTGGCTCAG
1492R 22 TACGGYTACCTTGTTACGACTT
533F 19 GTGCCAGCAGCCGCGGTAA
805R 20 GACTACCAGGGTATCTAATC

If there are Universal Primers not listed in the table, please contact our laboratory by phone or email for inquiries.

Strain Identification


Advantages of Strain Identification Service :
1. Genetic identification methods analyze bacterial 16S and fungal ITS, capable of identifying at the "species" level.
2. Both agar plates and liquid cultures of individual strains are applicable.
3. Reports are generated within 5 working days upon sample receipt.
4. A dedicated data management system is available for customers to download files.