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NGS FAQ

Ribo-seq

Ribo-seq is used to study translation at a genome-wide level. It enables researchers to examine ribosome positioning on mRNA, translation start sites, translational efficiency, and compare this data with other gene expression data to assess translational levels. This provides a deeper understanding of protein synthesis dynamics and finds applications in studying gene regulation, disease mechanisms, and drug development.

Ribo-seq specifically captures actively translated mRNA regions by ribosomes rather than the entire cellular RNA.

It offers a more direct measurement of translation compared to traditional RNA-seq, which measures overall mRNA abundance.

Yes, Ribo-seq can be employed to investigate translation in specific cell types or under specific conditions.

It's a versatile technique that allows an in-depth understanding of translational regulation in different biological contexts.

Ribo-seq can be applied to non-model organisms, but the suitability might be influenced by the availability of a reference genome or transcriptome.

In cases without suitable references, de novo assembly can reconstruct transcripts from sequencing data for analysis.

Tri-Ligo Oligo Synthesis

The advantages of Tri-Ligo Oligo synthesis :
1. High-efficiency synthesis speed
2. High-quality quality management
3. Reasonable price and discount
4. Insistence on Made in Taiwan

1. What is the purpose of Ribo-seq ?

2. How does Ribo-seq differ from traditional RNA-seq ?

3. Can Ribo-seq be used to study translation in specific cell types or conditions ?

4. Is Ribo-seq applicable to non-model organisms ?

Tri-Ligo Oligo Synthesis